First Report of Crown and Root Rot Caused by <i>Pythium aphanidermatum</i> on Tomato in Italy

HomePlant DiseaseVol. 105, No. 7First Report of Crown and Root Rot Caused by Pythium aphanidermatum on Tomato in Italy PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ItalyG. Gilardi, G. Tabone, V. Guarnaccia, A. Garibaldi, M. L. GullinoG. GilardiCentre Competence for Innovation the Agro-Environmental Sector (AGROINNOVA), University Torino, 10095 Grugliasco, ItalySearch more papers this author, TaboneCentre GuarnacciaCentre ItalyDISAFA, GaribaldiCentre Gullino†Corresponding author: Gullino; E-mail Address: [email protected]://orcid.org/0000-0002-7706-1915Centre author AffiliationsAuthors Affiliations Gilardi1 Tabone1 Guarnaccia1 2 Garibaldi1 Gullino1 † 1Centre 2DISAFA, Published Online:1 Aug 2021https://doi.org/10.1094/PDIS-10-20-2246-PDNAboutSectionsView articlePDFPDF Plus ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleDuring May June 2020, symptoms crown root rot were observed Solanum lycopersicum plants (cvs. Meneghino Vortex), grown a plastic tunnel Cuneo province (northern Italy). The first developed 40 60 days after planting, with air temperatures ranging from 13 29°C (minimum maximum night/day). collar stem 20 30% out 600 had brown discoloration, water-soaked, soft symptoms. Affected stunted quickly wilted, showing an extensive rot. Tissue fragments 1 mm2 excised margins lesions plants, dipped 1% sodium hypochlorite (1 min), rinsed sterile water, plated potato dextrose agar (Merck, Germany) 25 mg/liter streptomycin sulfate BNPRA semiselective medium oomycetes (Masago et al. 1977). Plates incubated under constant fluorescent light at 22°C 3 abundant aerial mycelium both media. Two isolates selected as representative (34-20V1; 34-20V2) V8 (vegetable mix, 300 g; agar, 15 CaCO3, 1.5 distilled liter); they showed aseptate hyphae, 3.8 9.9 µm (6.2 mean, n = 30) wide. Oogonia globose, smooth 16.8 29.4 (23.5 diameter. Antheridia barrel-shaped, oospores 15.3 26.1 (21.8 These morphological characters allowed identifying microorganism sp. (Spencer 2005). internal transcribed spacer region rDNA 34-20V1 34-20V2 was amplified using primers ITS1/ITS4 sequenced. A BLAST analysis (Altschul 1997) 801-bp sequences obtained (GenBank accession no. MW131326) MW131327) 99.9% homology (800/801 nucleotides) sequence strain CBS 118.80 AY598622). Pathogenicity tests performed twice 30-day-old (cv. Meneghino) 12-liter pots, containing steam-disinfested peat substrate, infested g/liter wheat hemp kernels (2:1 v/v) colonized P. aphanidermatum. Ten per pot planted three pots filled medium, same number noninfested substrate control. Pots kept completely randomized greenhouse 22 26°C. 4 7 planting; 17 after, 50 80% dead. Control remained healthy. consistently reisolated lesions. is pathogen wide host range (Farr Rossman 2020), it previously northern Capsicum annuum (Garibaldi 2014). important agent tomato worldwide 2020). It may also cause severe damage soilless (Stanghellini Russell 1971) or field conditions following flooding (Le 2003), particularly critical warm (Kläring 2001). To our knowledge, report presence Italy. spread disease could serious considering importance crop Italy.The author(s) declare no conflict interest.References:Altschul, S. F., 1997. Nucleic Acids Res. 25:3389. https://doi.org/10.1093/nar/25.17.3389 Crossref, ISI, Google ScholarFarr, D. Rossman, Y. 2020. Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. https://nt.ars-grin.gov/fungaldatabases/. ScholarGaribaldi, A., 2014. Plant Dis. 98:854. https://doi.org/10.1094/PDIS-11-13-1148-PDN Link, ScholarKläring, H.-P., 2001. Acta Hortic. 548:235. https://doi.org/10.17660/ActaHortic.2001.548.26 ScholarLe, H. T., 2003. Commun. Agric. Appl. Biol. Sci. 68:463. ScholarMasago, H., 1977. Phytopathology 67:425. https://doi.org/10.1094/Phyto-67-425 ScholarSpencer, 2005. I.M.I. Descr. Fungi Bact. 1613:1. ScholarStanghellini, E., Russell, J. 1971. Prog. Ariz. 23:15. ScholarFunding: Funding provided Fondazione Cassa Risparmio Cuneo, Agroalimentare 4.0, SFIDA Project ‘Low environmental impact management strategies horticultural sector’.The interest.DetailsFiguresLiterature CitedRelated Vol. July 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionLeaf spot Zizyphus mauritiana caused Colletotrichum spp. (J. Shu al.). Photo credit: Q. Li. Symptoms twig dieback tea Fusarium solani (A. K. Pandey Pandey. Metrics Downloaded 285 times Article History Issue Date: Oct 2021Published: 2021Accepted: Feb 2021 Page: 2022 Information© American Phytopathological SocietyFundingFondazione CuneoKeywordsoomycetesvegetablesepidemiologyThe interest.